In order to measure transcript abundance to collect data for my project, I need to extract RNA from frozen diatom cells. These diatom cells remain "frozen in time" from my experimental assays, which expose lines of diatoms to ammonium. By freezing the cells in liquid nitrogen, I can later extract their RNA and get a good idea of what the transcript abundance was at that time.
To find out more about my experimental assays click on the link up there (which is also here) and then watch my video down below about RNA extractions. These videos together describe some of the most important work for my project!
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